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H4502
Sigma
Hexokinase from Saccharomyces cerevisiae
Type F-300, lyophilized powder, ≥130 units/mg protein (biuret)
| Synonym: | ATP: |
| CAS Number: | 9001-51-8 |
| Enzyme Commission (EC) Number: | 2.7.1.1 ( BRENDA | IUBMB ) |
| EC Number: | 232-611-5 |
| MDL number: | MFCD00131321 |
Description
| Quality | A mixture of isoenzymes. |
| Reconstitution | Reconstitute with water or citrate buffer, pH 7.0. Studies have shown excellent stability during repeated freezing and thawing over a period of at least 30 days. |
| Unit Definition | One unit will phosphorylate 1.0 μmole of |
| Physical form | Lyophilized powder containing approx. 15% sodium citrate |
| Biochem/physiol Actions | Catalyzes the phosphorylation of The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C). Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively. Molecular Weight: ~ 54 kDa (monomer) ~110 kDa (dimer) Optimal pH: 7.5 to 9.0 Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds. Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate) |
Properties
| type | Type F-300 |
| form | lyophilized powder |
| purified by | crystallization |
| anion traces | sulfate (SO42-): free |
| foreign activity | ATPase, myokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucose isomerase ≤0.01% |
| Gene Information | bakers yeast ... HXK1(850614), HXK2(852639) |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| Reference | Kaji, et al. Ann. N.Y. Acad. Sci. 94, 798, (1961) Abstract |
| Bergmeyer, H., Grassl, H., and Walter, H-E., Bergmeyer, H.U., ed. Meth. Enzym. Anal. 3rd ed., 2, 222, (1983) |
